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Fig. 2 | Cardio-Oncology

Fig. 2

From: 14,15-Dihydroxyeicosatrienoic acid, a soluble epoxide hydrolase metabolite in blood, is a predictor of anthracycline-induced cardiotoxicity – a hypothesis generating study

Fig. 2

DOX treatment induced BNP expression and cell hypertrophy in H9c2 rat cardiomyocytes. Cells were collected at 2 h, 6 h and 24 h with 2 h pre-treatment of DMSO (0.01%) for controls or 1 µM DOX (Sigma) dissolved in DMSO (0.01%). A Levels of BNP mRNA assessed with rat specific BNP primers and normalized by β-actin mRNA in duplicate. B Cell area measured using light microscopy and Image J software. C Representative cell images obtained by light microscopy. D Cell viability measured by the MTT assay. Results were expressed as mean ± SD. *p < 0.05 compared to cells not treated with DOX using an independent T-test. The assays were performed in three replications using 4–6 plating for each replication

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Cardio-Oncology

ISSN: 2057-3804

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