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Fig. 4 | Cardio-Oncology

Fig. 4

From: 14,15-Dihydroxyeicosatrienoic acid, a soluble epoxide hydrolase metabolite in blood, is a predictor of anthracycline-induced cardiotoxicity – a hypothesis generating study

Fig. 4

Plasma levels of 14,15-DHET in rats following DOX treatment. Dox (3 mg/kg/week) was injected in female Sprague–Dawley rats (n = 12) whereas the same volume of saline was injected in control rats. A Body weight and percent of weight gain was assessed everyday over the course of the experiment. Rats were euthanized 48 h (no recovery) or after 2 weeks (2 wks recovery) of injections with saline (control) or 3 mg/kg/week DOX. Hearts were collected and RNA was isolated with TRIzol methodology and mRNA was assessed with rat specific B BNP, and C MPO primers in duplicate. Results were normalized by β-actin mRNA. Plasma was also collected after euthanized, extracted with ethyl acetate, and levels of 14, 15-DHET D and 20-HETE E were assessed by ELISA assay. Results were expressed as a mean ± standard deviation (SD). *p < 0.05 and **p < 0.01

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Cardio-Oncology

ISSN: 2057-3804

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