Fig. 7

Uptake and localization of FITC-Ac-SDKP by HMVEC-Cs: Human cardiac microvascular endothelial cells (HMVEC-Cs) were treated with FITC-labeled Ac-SDKP and scrambled peptide immediately prior to radiation, and the cells were incubated for 4 h. a: Representative confocal image showing FITC-labeled peptide (green) localized in to the non-irradiated (control) endothelial cells; b: DAPI-stained nuclei (blue), and c: Merged FITC and DAPI images. e-g shows localization of FITC-Ac-SDKP in irradiated HMVEC-Cs. i-k shows weak, non-specific localization of scrambled peptide, FITC-Ac-KDPS, in irradiated HMVEC-Cs. d, h and l compares FACS results for FITC-Ac-SDKP in non-irradiated cells (30% uptake), FITC-Ac-SDKP in irradiated cells (32% uptake), and FITC-Ac-KDPS (scrambled peptide) in irradiated cells (9% uptake), respectively. The gating was set with FL1-H (FITC) positive cell population in green and negative cells in blue. m and n: Graphs showing representative irradiated unstained population used as gating reference (m) and dose-dependency of FITC-Ac-SDKP uptake by endothelial cells (n). N = 3 in each treatment. Scale bar:50 μm, magnification × 400