Table 1 Primers used in the quantitative real time-PCR
Gene | Forward Primer 5′to 3′ | Reverse Primer 5′to 3′ | Length mRNA (bp) |
|---|---|---|---|
Claudin-1 | GGCCAGGCTCTCTTTACTGG | AGAGGTTGTTTTCCGGGGAC | 93 |
Claudin-3 | AAGGTGTACGACTCGCTGCT | AGTCCCGGATAATGGTGTTG | 247 |
Claudin-5 | GGGCGTCCAGAGTTCAGTTT | TAACTTGCCTCGGAGTCTGC | 213 |
JAM-2 | GGCGACTTTAAAGACCGTGC | AGTTGCGGGCTTCACAGTAA | 412 |
Occludin | GGATTGAGCCCGAGTGGAAA | GAGGTAGCACCACGTTGGAA | 400 |
ZO-1 | GGGGCCTACACTGATCAAGA | GGTCTCTGCTGGCTTGTTTC | 370 |