Fig. 8

In vivo co-localization of FITC labeled Ac-SDKP with vWF staining: FITC-Ac-SDKP uptake in coronary endothelial cells were examined after 4 h of injections in mice with or without irradiation. Confocal images a-c show FITC-stained intracellular Ac-SDKP in green, Phycoerythrin (PE) labelled vWF as the endothelial marker in red and DAPI stained nuclei in blue. a shows co-localization of peptide with vWF in arteriolar wall in non-irradiated (control) mouse. Panel b represents tissue sections from radiation exposed mouse with arterial FITC-internalization in arteriolar endothelial cells. c shows scrambled peptide image. Scale bar: 50 μm, magnification × 400